Biological Sciences and Analytical Chemistry. HPLC analysis of glycosaminoglycan chains in tissue.

Abstract

Glycosaminoglycan chains were prepered from bovine heart by succesive digestion with protease and endo-β-xylosidase. The glycosaminoglycan chins were then labeled with a fluorescent reagent, 2-aminopyridine, by reductive amination. The resulting pyridylamino-glycosaminoglycans, including hyaluronic acid, heparan sulfate, chondroitin sulfate/dermatan sulfate and heparin, were separated by ion-exchange high-performance liquid chromatography using a TSK-gel SAX analytical column with a sensitivity limit in the picomol range.