Complement Fixation with Brunhilde and Lansing Poliomyelitis Viruses Propagated in Tissue Culture.

Abstract

Antigens which gave specific complement fixation were concentrated from the supernatant fluids of tissue cultures infected with poliomyelitis viruses (Lansing and Brunhilde strains) by a method of ultrafiltration. Their anticomplementary activity was abolished by heating. The drop method of Fulton and Dumbell was used and offered a practical way of performing the tests with minimal amounts of antigen. Specific antibodies were demonstrated in the sera of hyperimmunized monkeys as well as in sera of poliomyelitis patients.