Immunocytochemical localization of glutathione-peroxidase (GSH-PO) in rat peritoneal macrophages.

Abstract

In order to confirm the relationship between prostaglandin synthesis and lipid peroxidation in the rat peritoneal macrophages, we have studied the immunocytochemical localization of glutathione-peroxidase (GSH-PO) in the rat peritoneal macrophages under zymosan, phorbol myristate acetate (PMA), ionophore A23187, testosterone, parabromophenacyl bromide (PBPB) and BW 755C treatment. In the untreated peritoneal macrophages, GSH-PO was predominantly observed in cytosol. In zymosan, PMA, ionophore A23187 and testosterone-elicited peritoneal macrophages, GSH-PO was mainly detected in cytosol as large granules. In contrast, the intensity of the GSH-PO staining in PBPB-elicited peritoneal macrophages were weaker than that of other experimental groups. In BW 755C-elicited peritoneal macrophages, GSH-PO was clearly observed in cytosol. Based on our findings, it is strongly suggested that GSH-PO in peritoneal macrophages may be playing an important role in reduction of lipid peroxides such as arachidonic acid hydroperoxides induced in the process of arachidonic acid cascade.