Biochemical Society Transactions · 1988 · 35 citations · 0 references
Life SciencesGeneticsMolecular GeneticsGene ExpressionGenetic EngineeringMicrobial GeneticsFungal GeneticsHeterologous Protein ExpressionYeast BiologyYeast GeneticsComplementationEnzyme Expression In FungiEnzyme Expression In YeastGenetic Linkage AnalysisRecombinant Protein Expression In FungiRegulatable Promoter SystemsRenewable Carbon SourcesYeast Gene Expression Regulation
The advantages of Hansenula polymorpha as a new yeast expression system are discussed in terms of the powerful and regulatable methanol oxidase promoter and the organism's ability to grow on cheap carbon sources. The development of techniques for conventional genetic analysis is described. A total of 218 mutants have been assigned to 62 complementation groups, three genes have been found to be linked forming the first linkage group in this organism. Methods for molecular transformation have been developed allowing the expression of heterologous genes. The disruptive integration and expression of the neomycin phosphotransferase is described.