Human Vaccines & Immunotherapeutics · 2012 · 12 citations · 20 references
Life SciencesCell BiologyImmunologyCellular ImmunologyInflammationInnate ImmunityCytokine BiologyCytokine RegulationCytokine SignalingDendritic Cell BiologyDendritic Cell RegulationImmune Cell PhysiologyDendritic Cell Cytokine ProductionDendritic Cell Cytokine SignalingDendritic Cell Inflammatory ResponsesDendritic Cell PhagocytosisDendritic Cell SignalingDendritic Cell-mediated Antigen PresentationFlow Cytometry ImmunophenotypingMhc Class Ii Molecules
The aim of present research is to analyze the detailed changes of dendritic cells (DCs) induced by pidotimod(PTD). These impacts on DCs of both bone marrow derived DCs and established DC2.4 cell line were assessed with use of conventional scanning electron microscopy (SEM), flow cytometry (FCM), transmission electron microscopy (TEM), cytochemistry assay FITC-dextran, bio-assay and enzyme linked immunosorbent assay (ELISA). We demonstrated the ability of PTD to induce DC phynotypic and functional maturation as evidenced by higher expression of key surface molecules such as MHC II, CD80 and CD86. The functional tests proved the downregulation of ACP inside the DCs, occurred when phagocytosis of DCs decreased, with simultaneously antigen presentation increased toward maturation. Finally, PTD also stimulated production of more cytokine IL-12 and less TNF-α. Therefore it is concluded that PTD can markedly exert positive induction to murine DCs.
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Kensuke Inaba, Muneo Inaba, Nikolaus Romani et al. · The Journal of Experimental Medicine · 1992 · 3.8K citations · Full text