Enzymatic Estimation of Urinary Steroids.

Abstract

A novel method for the sensitive and specific microestimation of steroids has been described. This method depends upon the selective oxidation or reduction of respectively hydroxy- and ketosteroids by highly purified hydroxysteroid dehydrogenases of bacterial origin. The method permits the estimation of 3a-hydroxysteroids, 3β-(and 17β-) hydroxysteroids, 3-ketosteroids and 17-ketosteroids.