Izhakiella capsodis gen. nov., sp. nov., in the family Enterobacteriaceae, isolated from the mirid bug Capsodes infuscatus

Abstract

Gram-stain-negative, oxidase-negative, facultatively anaerobic, motile, rod-shaped, non-pigmented bacterial strains (N6PO6T, N8PO1 and N8PI1) were isolated from the mirid bug Capsodes infuscatus captured on Asphodelus aestivus plants. The 16S rRNA gene sequences of the strains shared 94.7–95.7 % similarity with species of the genus Pantoea and 95.6 % or less with species from other genera in the family Enterobacteriaceae. A polyphasic approach that included determination of phenotypic properties and phylogenetic analysis based on 16S rRNA, rpoB, gyrB and atpD gene sequences supported the classification of strains N6PO6T, N8PO1 and N8PI1 as representing a novel species of a new genus in the family Enterobacteriaceae. Strain N6PO6T, and the two reference strains of the novel species, grew at 1–37 °C, and in the presence of NaCl (up to 7.5 %, w/v) and sucrose (up to 60 %). Their major cellular fatty acids were C16 : 0, C17 : 0 cyclo, C18 : 1ω7c, summed feature 2 (C14 : 0 3-OH and/or iso-C16 : 1 I) and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). The DNA G+C content of strain N6PO6T was 49.9 mol%. On the basis of phenotypic properties and phylogenetic distinctiveness, the mirid bug isolates are classified as representing a novel species in a new genus Izhakiella, in the family Enterobacteriaceae, for which the name Izhakiella capsodis gen. nov., sp. nov. is proposed. The type strain of Izhakiella capsodis is N6PO6T ( = LMG 28430T = DSM 29293T).