Growth of Animal Tissue Cells in Artificial Media.

Abstract

1. The difference between growth-promoting substances and accessory growth substances and between their different modes of action is pointed out. 2. From dialysates from embryo juice, preparations may be obtained which completely restore the ability of the dialysed culture media to induce normal growth of the tissue cultures. They may be prepared free from organic phosphates and glutamine. 3. A synthetic mixture is described which has the same properties of restoring the dialysed media. The most important of its components seem to be glutamine and fructose-diphosphate.