Application of borate ion-exchange mode high-performance liquid chromatography to separation of glycosides: Saponins of ginseng, Sapindus mukurossi Gaertn. and Anemone rivularis Buch.-Ham.

Abstract

High-performance liquid chromatography (HPLC) of borate complexes on a basic ion-exchange column was investigated for the separation of saponins of Ginseng, Sapindus mukurossi and Anemone rivularis, as well as other synthetic glycosides including steviol glycosides. It was found that HPCL in this mode is effective for analysis and preparative separation of a variety of glycosides, especially isomeric glycosides, containing xylopyranosyl, arabinofuranosyl or arabinopyranosyl units.