Fluorescent Antibody and Complement-Fixation Tests of Agents Isolated In Tissue Culture from Measles Patients.

Abstract

Agents isolated in monkey kidney tissue cultures from 3 cases of measles were identified by immunologic tests with convalescent-phase measles serum. Measles antigen was detected in infected cells by the indirect fluorescein-labeled antibody technic before specific complement-fixation antigen appeared in the fluid phase. Specific fluorescence due to the presence of measles antigen was noted in the nuclei as well as in the cytoplasm of some cells from infected cultures.