Distribution of Glycoproteins in Normal Human Plasma.

Abstract

Five-ml samples of normal, human plasma have been separated by a low temperature-low salt-ethanol procedure into 4 primary fractions, I + III, II, IV + V, and VI. The concentrations of protein-bound carbohydrate (hexose) and of protein in whole plasma and the 4 primary fractions and the protein-bound carbohydrate content of sero-mucoid were determined. Fraction IV + V, of the primary fractions, contained the greatest amount of protein-bound carbohydrate and of protein. The percentage of protein-bound carbohydrate with respect to protein was highest in Fraction VI.