Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE · 2016 · 10 citations · 11 references
EngineeringBiomedical EngineeringBiophysicsBiomedical OpticsBiophotonicsDiagnostic ImagingBiomedical Laser ApplicationsLaser OpticsLaser-tissue InteractionLight-tissue InteractionOptical ImagingPhotoacoustic ImagingBeam Pulse DurationExcitationFast Image AcquisitionLaser Pulse DurationPhotoacoustic Excitation SourcesPhotoacoustic Imaging InstrumentationPhotoacoustic Imaging SystemsPhotoacoustic Microscopy
Two custom fibre lasers have been developed. One is designed for widefield photoacoustic tomography (PAT) and uses a custom drawn large core diameter fibre (100μm) to provide high pulse energies (5mJ). It also provides a variable pulse repetition frequency (100Hz-400Hz) and pulse duration (10-150ns) and is compact (of comparable dimensions to a desktop PC) and does not require external water cooling. This system was used to acquire in vivo images of the subcutaneous microvasculature in the human palm. The second laser is designed for Optical Resolution Photoacoustic Microscopy (OR-PAM) and provides a high quality beam (M2<1.1), pulse energies >1μJ with a pulse repetition frequency (PRF) up to 2MHz, and a 532nm emission wavelength. The high PRF of this laser was exploited for ultra-fast image acquisition. The compact size and enhanced functionality of these lasers offers a major opportunity to facilitate the translation of photoacoustic imaging to practical applications in medicine and biology.
11
High-speed label-free functional photoacoustic microscopy of mouse brain in action
Junjie Yao, Lidai Wang, Lihong V. Wang et al. · Nature Methods · 2015 · 722 citations · Full text
Edward Zhang, Jan Laufer, Paul C. Beard · Applied Optics · 2008 · 569 citations