Display of GPI‐anchored anti‐EGFR nanobodies on extracellular vesicles promotes tumour cell targeting

Abstract

We show that nanobodies can be anchored on the surface of EVs via GPI, which alters their cell targeting behaviour. Furthermore, this study highlights GPI-anchoring as a new tool in the EV toolbox, which may be applied for EV display of a variety of proteins, such as antibodies, reporter proteins and signaling molecules.