Revised Method for Determination of Aspirin and Salicylic Acid in Human Plasma by High Pressure Liquid Chromatography

Abstract

Abstract The method for the analysis of aspirin and salicylic acid in human plasma has been updated to include advances in column technology, extraction procedures and absorbance detection. Aspirin and salicylic acid are extracted from acidified plasma into an organic solvent system containing internal standard. Following controlled evaporation under partial vacuum of the organic extract, the dried down-residue is reconstituted with mobile phase. Chromatography is ion suppression reverse phase on a 5 μm O.D.S. column with detection by absorbance at 237 nm and optional fluorescence. Concentration of aspirin as low as 0.20 μg/ml and salicyclic acid as low as 0.50 μg/ml can be quantitated.