Epigenetic Regulation in Early Childhood: A Miniaturized and Validated Method to Assess Histone Acetylation

Abstract

We developed a ChIP method allowing reliable assessment of H3/H4 acetylation using 1 × 105 cells only. Practical application of this assay demonstrated an association between high maternal folate exposure and increased histone acetylation, corresponding to a more transcriptionally permissive chromatin status in the promoter regions of some Th2-related genes.