Boeck and Drbohlav Locke egg serum medium for detection of Blastocystis hominis

Abstract

Objective To study Blastocystis hominis infection, positivity by using Boeck and Drbohlav Locke egg serum medium (LE) cultivation and Jones’ medium. Design Cross-sectional study. Setting Bann Kew Savag Mai, Mae Chame District, Chiang Mai Province and Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. Materials One hundred and fifty-eight stool specimens from hill-tribe children, aged 1-18 years old. Methods All 158 stool specimens were cultivated in Jones’ and LE media, transported to the laboratory and examined for B.hominis under the microscope. The parasites were detected after a 72 hr incubation at 37°C. Each stool sample was also identified for B.hominis by simple smear and concentration techniques. Results The prevalence rate of B.hominis was 0.91% and 1.09%, examined by simple smear and formalin ether concentration technique, respectively. The Jones’ medium demonstrated 22.15% parasite positivity. The LE medium showed a high efficiency of organism detection with 43.67% parasite positivity. นพนธตนฉบบ