Methods in molecular biology · 2013 · 26 citations · 35 references
Life SciencesBiotechnologyMolecular BiologyGene ExpressionMolecular BiotechnologyProtein EngineeringExpression Vector DesignGene Library ConstructionMolecular Cloning WorkflowsProtein Expression EngineeringRecombinant Plasmid ConstructionRecombinant Protein ExpressionBacterial Expression VectorsCloningEscherichia Coli Expression SystemsExpression Library ConstructionExpression Vector CloningParallel ConstructionRecombinant Expression Systems
In-Fusion™ cloning is a flexible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in Escherichia coli of constructs generated by In-Fusion™ is also outlined.
35
Enzymatic assembly of DNA molecules up to several hundred kilobases
Daniel G. Gibson, Lei Young, Ray-Yuan Chuang et al. · Nature Methods · 2009 · 10.9K citations
Bin Zhou, Matthew E. Donnelly, Derek T. Scholes et al. · Journal of Virology · 2009 · 679 citations · Full text