The Continuing Development of E. coli as a Heterologous Host for Complex Natural Product Biosynthesis

One-step inactivation of chromosomal genes in <i>Escherichia coli</i> K-12 using PCR products

Kirill A. Datsenko, Barry L. Wanner · Proceedings of the National Academy of Sciences · 2000 · 15.2K citations · Full text

Life Sciences, Genetics, Molecular Genetics +17

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

F. William Studier, Barbara A. Moffatt · Journal of Molecular Biology · 1986 · 6.4K citations

Life Sciences, Microbiology, Molecular Biology +9

A new enzyme superfamily — the phosphopantetheinyl transferases

Ralph H. Lambalot, Amy M. Gehring, Roger S. Flugel et al. · Chemistry & Biology · 1996 · 857 citations · Full text

Life Sciences, Biochemistry, Molecular Biology +12

Biosynthesis of Complex Polyketides in a Metabolically Engineered Strain of <i>E. coli</i>

Blaine A. Pfeifer, Suzanne J. Admiraal, Hugo Gramajo et al. · Science · 2001 · 760 citations

Life Sciences, Biotechnology, Molecular Biology +17

Characterization of Sfp, a <i>Bacillus subtilis</i> Phosphopantetheinyl Transferase for Peptidyl Carrier Protein Domains in Peptide Synthetases

Luis E. N. Quadri, Paul H. Weinreb, Ming Lei et al. · Biochemistry · 1998 · 701 citations

Life Sciences, Biochemistry, Molecular Biology +12