The Temperature Relations of Clostridium Botulinum, Types A and B

Abstract

Ten strains of Cl. botulinum, type A, and 10 of type B have been studied at 12 temperatures between 10 and 50°C., and rates of growth measured nephelometrically on sealed cultures. Growth proceeded from spore inocula at temperatures from 15 through to 42.5°C., but not at 12.5 or 45°C. When young, actively growing cultures were transferred to temperatures outside the range permitting spore germination, rates of growth were measured at 12.5, 45, and 47.5°C. After transfer to 10 or 50°C. no sustained growth was observed