PubMed · 1973 · 30 citations · 6 references
Life SciencesImmunologyPharmacologyAllergyHypersensitivityImmunophysiologyAllergic ConditionsAllergic DiseaseAllergic Skin DiseasesAnaphylaxisHistamine ReleaseIge-mediated AllergyAllergen-induced Histamine ReleaseAnaphylactic Histamine ReleaseBasophil Histamine Release TestHistamine Release AssaysHistamine Release InhibitionHistamine Release KineticsHistamine Release Measurement
Abstract An in vitro microassay is described for studying allergic release of histamine from human leukocytes. The procedure takes advantage of a very sensitive enzymicisotopic assay for histamine which measures the histamine in a 20 μl sample volume; the assay can detect 0.1 ng. histamine. Each leukocyte reaction is run in a total volume of 0.1 ml. An antigen dose-response curve requires only 1 ml. of donor blood. This method is particularly advantageous for studies with children and when repetitive bleeding of individual donors is required.
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