Clinical Chemistry · 1959 · 47 citations · 0 references
Life SciencesBiochemistryPhysiologyClinical BiochemistryEnzymologyMetabolismClinical EnzymologyEnzyme CharacterizationEnzyme KineticsActivity-temperature CouplingEnzyme Kinetics AssaysEnzyme Kinetics In DiagnosticsIsozyme AssaysSerum Enzyme Activity MeasurementSubstrate InhibitionTemperature Dependence Of EnzymesTemperature Effects On Enzyme Activity
Abstract 1. Factors influencing the isocitric dehydrogenase (ICD) activity of serum were studied. Substrate inhibition by high concentrations of isocitrate was observed. 2. The temperature-activity curve of serum ICD was determined. The importance of controlling the temperature of the reaction was stressed. 3. Using this information, a method of measuring the ICD activity in 0.2 ml. of serum is described. 4. The range of ICD activity in 50 healthy males was 30 to 192 units with a mean of 90 units and a standard deviation of ± 34.3 units.