INVITRO ANTI-INFLAMMATORY ACTIVITY OF METHANOL EXTRACT OF ENICOSTEMMA AXILLARE

Abstract

Methanol extract of whole plant of Enicostemma axillare (Family: Gentianaceae) was assessed for its anti-inflammatory activity by invitro methods. Invitro anti-inflammatory activity was evaluated using albumin denaturation assay, proteinase inhibitory activity, membrane stabilization, and anti-lipoxygenase activity at different concentrations. Aspirin, Diclofenac sodium, Indomethacin were used as standard drugs. The results showed that Enicostemma axillare Methanol Extract (EAME) at a concentration range of 100-500µg/ml significantly (p 0.05) activity. Heat induced haemolysis of erythrocyte was significantly (p<0.05) inhibited at the concentration of 400 and 500µg/ml. Hypotonicity induced haemolysis and lipoxygenase activity were significantly (p<0.01) inhibited at the concentration range of 200-500µg/ml and 400, 500µg/ml respectively. The results obtained in the present study indicate that methanol extracts of Enicostemma axillare can be a potential source of anti-inflammatory agents.