Direct Isolation of Exosomes from Cell Culture: Simplifying Methods for Exosome Enrichment and Analysis

Abstract

Flow cytometric analysis of exosomes isolated with magnetic beads. SW480 derived exosomes (B and C) and Jurkat derived exosomes (D and E) were immunoisolated from pre-enriched exosome samples using magnetic beads coated with anti-human CD9 or CD81. Exosomes were stained for exosomal antigens CD9 (B and D) and CD81 (C and E) followed flow cytometric analysis and presented as Median Fluorescence Intensity (MFI). A) Scatter plot of exosomes isolated with anti-CD81 and labelled for CD9 to demonstrate gating. Shown is one representative result out of three independent experiments.