Gene transfer in Indian major carps by electroporation

Abstract

The rainbow trout growth hormone gene has been successfully tranferred into three species of Indian major carps rohu (Labeo rohita), mrigal (Cirrhinus mrigala) and catla (Catla catla) through electroporated sperm cell. At 0.5kV/cm (25μF capacitance, a resistance, and 2 pulses) the gene transfer efficiency was 25% for rohu, 23% for mrigal and 13% for catla. This is the first report on gene transfer in Indian major carps.