Production, Optimization and Characterization of Fungal Cellulase for Enzymatic Saccharification of Lignocellosic Agro-waste

Abstract

A B S T R A C T Cellulases are a group of hydrolytic enzymes and are capable of degrading lignocellulosic materials. Cellulases have wide range of applications. This work focuses on factors relevant for improvement of enzymatic hydrolysis of rice straw by using isolated fungal strain from compost pit. Different cultural conditions were examined to assess their effect in optimizing enzyme production as well as for characterization of enzyme after partial purification. In comparison with WR (White rot fungi), our isolated strain 2b reports higher production of cellulases. K e y w o r d s Fungal cellulase, Lignocellulolytic agrowaste, Enzymatic saccharification