Zeitschrift für Naturforschung C · 1986 · 60 citations · 0 references
Life SciencesPlant BiologyPlant SciencesChromatographic AnalysisPlant BiochemistryPlant PhysiologyAnalytical ChromatographyChromatographic PurificationChromatographic SeparationChromatographyEnzyme IsolationPreparative ChromatographyActive Compound IsolationPigment Isolation
Abstract Native (124 kDa) phytochrome has been isolated from etiolated oat seedlings in 25% yield. The procedure includes only one chromatographic step namely a hydroxylapatite column, besides several centrifugation steps. Large scale preparations with 3 kg of fresh tissue are completed within 16 h. The purity of isolated phytochrome is as good as or even better than that of previous preparations as deduced from SDS gelelectrophoresis and from the absorbance ratio A 667 / A 280 = 0.99