PHYTOCHEMICAL SCREENING AND DETERMINATION OF ANTI-BACTERIAL AND ANTI-OXIDANT POTENTIAL OF Glycyrrhiza glabra ROOT EXTRACTS

Abstract

The present study was undertaken to explore the phytochemical screening, anti-bacterial and anti-oxidant activities of the hydro-methanolic root extract of Glycyrrhiza glabra (Leguminosea) using standard screening methods, disc diffusion and de-oxyribose methods respectively. Different degrees of effect was noticed incase of different methods of study. In phytochemical screening, Glycyrrhiza glabra showed presence of secondary metabolites flavonoids, saponins, glycosides, terpenoids etc. It also showed potent antibacterial activities against almost all the test organisms. It exhibited highest sensitivity against Shigella flexineri with the zone of inhibition 10 mm. The extracts possessed potent hydroxyl radical scavenging activity having IC50 value 80µg/ml (52.5±0.79) against the positive control standard Ascorbic acid having IC50 value 50µg/ml (51.11±0.66). Results denote the presence of hydroxyl radical scavenging principles in the extracts.