ANTIBACTERIAL, ANTIOXIDANT ACTIVITY AND GC-MS ANALYSIS OF Eupatorium odoratum

Abstract

major and 26 minor compounds were identified in methanol and aqueous extracts of Eupatorium odoratum by GC -MS analysis showing significant antibacterial, antioxidant and other prophylactic activities. Antibacterial properties of aqueous and organic extracts of different parts of E.odoratum against nine different bacterial strains are examined. All fractions of leaf and root have significant inhibitory activity against all bacterial pathogens tested. However, flowers and stem did not show any activity. Significant protective activities against different ailments are found due to the presence of different phenols, flavonoids, alcohol derivatives and unique compounds consist of 2,4-Bis(1-phenylethy)phenol, Monoethylhexyl phthalate, Hoslundin, 2,4,6-Tris-(1-phenylethyl)-phenol; dl-α-Tocopherol, Phytol, 1,2,4-oxadiazol-5-amine, 3-(4-amino-1,2,5-oxadiazol-3-yl)-N-(2- (4-methoxyphenyl), 1-Heptacosanol, Stigmasterol, γ-Sitosterol, Tetra-O-methylscutellarein, Neophytadiene, (35)-7-O-Methoxymethylvestitol, α- Amyrin, Methylcommate D and 4-Acetyl-3-hydroxy-2,6-dimethoxytoluene. Our results revealed that, experiments conducted on different parts of this plant according to the traditional usage and several compounds identified by GC-MS analysis are principal factors for significant antibacterial, antioxidant and other prophylactic activities.