Complete Sequences of the S-genes, Sd- and Sh-RNase cDNA in Apple

Abstract

The S -locus genes in the pistil (S-RNases) were cloned from the apple ( Malus × domestica Borkh.) cultivar Akane ( S -genotype SdSh from pollination analysis). The Sd- and Sh-RNase corresponded to S7- and S24-RNase, which have been cloned from `Idared' and `Braeburn', respectively. Sh-RNase was very similar to Sf- and Sg-RNases at the deduced amino acid-sequence levels (93%). We developed an S -allele specific polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis method for distinguishing the Sh from Sf and Sg , and the Sh -alleles of `Akane', `Touhoku 2', `Vista Bella', and `Worcester Pearmain' were identified. We also identified the S -allele genotypes of 16 apple cultivars.