The Effects of Trypan Blue on Chick Embryos Cultured <i>in vitro</i>

Abstract

ABSTRACT Chick embryos at the primitive-streak or early head-process and at the medullary-plate stages were treated with Trypan blue during culture in vitro. The dye produced many abnormalities such as malformation of somites, shortening of the axis, the formation of large blisters on the embryo, and delayed heart flexure. In some specimens notochord failed to form and a median somite strand was found in these cases.