A New Technique for the Cultivation of the Chick Embryo <i>in vitro</i>

Abstract

ABSTRACT A new technique is described for the cultivation of chick embryos in vitro. It consists essentially in supporting the explanted blastoderm on a piece of vitel-line membrane stretched across a glass ring. The nutrient medium is fluid albumen. Primitive streak blastoderms explanted by this technique: (a) frequently develop as far as the formation of a functional blood circulation, together with flexion and torsion of the embryo; (b) show normal expansion across the vitelline membrane; (c) develop at a normal rate until after the 20-somite stage.