Biosynthesis of Silver Nanoparticles from Dental Caries Causing Fungi Candida albicans

Abstract

A B S T R A C T The work is being carried out for the applications which would help in the prevention of human pathogens. We describe the synthesis of silver nanoparticles using fungi Candida albicans. A strong characteristic absorbance peak at around 400 nm was observed at different time intervals. Particle size analysis of these particles shows that they are 50-100 nm in range and also this paper deals with a thorough investigation on the characterization of the silver nanoparticles by UV Visible, XRD, SEM and FTIR analysis and result reveals that, the average grain size of the silver nanoparticles formed in the bioreduction process is determined by XRD pattern of the silver nanoparticles formed in the experiment and is estimated to be greater than 50 nm and spherical in shape. FTIR spectral analysis showed array of absorbance bands in 400 cm -1 -1500 cm -1 finally tested the biocompatibility by antimicrobial test against multi drug resistant human pathogens like Escherichia coli, Klebsiella sp, Salmonella sp, Pseudomonas sp and Staphylococcus aureus. The evaluation of agar diffusion test was made on the basis of zone of inhibition of bacteria around the test sample. The result reveals that the maximum antimicrobial activity was observed for Staphylococcus aureus. K e y w o r d s Silver nanoparticles, Candida albicans, Pathogens, Antimicrobial activity