DETERMINATION OF VANCOMYCIN BY USING RP-HPLC METHOD IN PHARMACEUTICAL PREPARATIONS

Abstract

A novel, simple and economic reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for the estimation of vancomycin in tablet dosage forms with high precision and accuracy. Sample separation was achieved on C18 column (250 X 4.6mm i.d., 5m) in isocratic mode using methanol, Acetonitrile (ACN) and 0.1% Ortho phosphoric acid (OPA) in the ratio (25:72:3) (v/v/v) as mobile phase, pumped in to the column at flow rate of 1 ml/min and the detection of eluent from the column was carried out using variable wavelength detector at 229 nm. The total run time was 7 min and the column was maintained at ambient temperature. The retention time of Vancomycin was 2.822 min. The standard curves were linear over the concentration range of 2-12 g/ml and the LOD and LOQ values for Vancomycin were 0.1 and 0.03 respectively. The recovery was found to be 99 percent and the % RSD of intraday and inter day precision was found less than 2%, respectively. The percentage amount of two different marketed tablet formulation of Vancomycin was found to be 99.5%.The method was validated as per ICH (International council of Hormonisation) guidelines. Validation studies demonstrated that the proposed RP-HPLC method is simple, specific, rapid, reliable and reproducible. The high recovery and low relative standard deviation confirm the suitability of the proposed method for the routine quality control analysis of Vancomycin in tablet dosage forms.