Analysis of Active Components of Trigona spp Propolis from Pandeglang Indonesia

Abstract

Propolis has been shown to have anticancer, antifungi and antimicrobial activity. This research was aimed to analyze the active compounds of propolis through fractination using the Thin Layer Chromatography (TLC) and column chromatography methods followed by determination of the antibacterial activity of each faction. The active agents from the fraction showing highest antibacterial activity were then determined by GC-MS technique. Resulted showed that the active agents were present in eight different fractions. Test on antibacterial activity against E. coli showed a fraction (denoted c fraction) has the highest antibacterial activity. GC-MS analysis showed that c fraction contained at least 24 compounds. The most abundance compound from c fraction was similar to 9, 19-cyclolanost-24-en-3-ol, (3.beta)-(CAS) or cycloartenol. This compound had retention time of 40.25 minute and area of 49.91% out of the total area. Keyword: propolis, Trigona spp, antibacterial agent, active compounds.