ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF THE TISSUE EXTRACT OF PERNA VIRIDIS LINNAEUS, 1758 (MOLLUSCA: BIVALVIA) FROM VERSOVA COAST, MUMBAI

Abstract

Objective: The study of marine organism for their bioactive potential, being a significant part of marine ecosystem has picked up the regularity in recent years with the growth recognition of their importance in human life as well as animals. Methods: In this present study methanol tissue extract of edible green mussel (P. viridis) was assayed for the antibacterial activity against six bacterial pathogens and antioxidant activities also determined. Results: The antibacterial activity of P. viridis tissue extract, showed maximum zone of inhibition (15mm) against Vibrio cholerae and minimum activity (5 mm) was observed in Klebsiella pneumoniae. Molecular size of green mussel protein was determined using Sodiumdodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). FTIR analysis reveals the presence of bioactive compounds signals at different ranges. The antioxidant activity of crude protein tissue extract from the P. viridis were measured in different system of assay such as DPPH assay (76.9%), total reducing power (27.8 μg), total antioxidant activity (174 μg), hydrogen peroxide scavenging (88.12 %) and nitrous oxide scavenging activity (62.5%) at 100μg/ml. Conclusion: crude tissue extract from P. viridis could be effectively used as alternative source of antimicrobial and antioxidant with subsequent health benefits.