PREPARATION AND CHARACTERIZATION OF IBUPROFEN LOADED TRANSFEROSOME AS A NOVEL CARRIER FOR TRANSDERMAL DRUG DELIVERY SYSTEM

Abstract

The goal of this study was to develop and evaluate the potential use of transfersome vesicles in the transdermal drug delivery of Ibuprofen. It was investigated by encapsulating the drug in various formulations of composed of various ratios of soya phosphatidylcholine ,span 80 and tween 80, prepared by lipid film hydration by rotatary evaporation method and evaluated for particle shape, size, zeta potential, entrapment efficiency (%EE),elasticity, stability, and in vitro skin permeation. The vesicles were spherical in structure as confirmed by Scanning Electron Microscopy and TEM, the vesicle size of best formulation for Span 80 and Tween 80 was 962 nm and 2250 nm respectively, and zeta potential (negatively charged) for Span 80 and Tween 80 was found to be -16.1 and -17.5 respectively. The %EE of ibuprofen in the vesicles was 47.8±2.2 and the elasticity of both increases with increase in surfactant conc. and were found to be 34.4±1.4 and 26.5±1.6. Stability studies for Transferosome were carried out for 5 weeks at 450C. In vitro skin permeation studies were carried by human cadaver skin using franz diffusion cell, and drug release after 24 hrs and flux was found 2.5824 and 1.9672 ug/cm2/hr respectively. Fourier Transform Infrared Spectroscopy (FT-IR) and Differential Scanning Calorimetry (DSC) analysis indicated that the application of transfersomes significantly disrupted the stratum corneum lipid. It is evident from this study that transfersomes are a promising prolonged delivery system for Ibuprofen and have reasonably good stability characteristics. This research suggests that ibuprofen loaded transfersomes can be potentially used as a transdermal drug delivery system.