Synthetic seed technology for encapsulation and regrowth of in vitro- derived Gypsophila paniculata L. shoot- tips

Abstract

Encapsulation of in vitro-derived shoot tips of Gypsophila in different concentrations of Naalginate dissolved either in MS-salts solution or sterilized distilled water (SDW) as solvent was investigated. The encapsulated shoot-tips were stored 30 and 90 days at 4°C and cultured either on MS basal medium or on MS-medium containing 0.5 mg/l each of NAA and BA. The highest frequency of shoot emergence and maximum number of shoots were recorded for beads encapsulated in 4% Na-alginate dissolved in MS- salt solution which stored 30 days and grown on medium containing BA and NAA. However, the root emergence and the percentage of root formation in shoots obtained by beads dissolved in SDW was higher than that derived from beads dissolved in MS salt solution especially with high levels of Na-alginate. Furthermore, the degree of vitrification was affected by Na-alginate concentration. The degree of vitrification was decreased from 100% to 20% by increasing the Na-alginate concentration from 2% to 4% dissolved in SDW. SDS-PAGE analysis of shoots produced from beads after 90 days storing demonstrated the similarity of produced plantlets in the protein profile. Finally, plants retrieved from the encapsulated shoot-tips coated in 4% Na-alginate were hardened off and successfully established in the growth chamber.