Production of α-amylase using banana waste by Bacillus subtilis under solid state fermentation

Abstract

Banana waste was used as a substrate for the produc tion of amylase by Bacillus subtilis using solid st ate fermentation. The various process parameters were othe incubation period, substrate concentr ation, pH and incubation temperature showed 24hrs, 50g, 7 pH and 35°C respectively. Peptone (0.2%) as a nitrogen sources showed maximum yield and the maximum enzyme activit y showed in presence of in organic nutrients magnes ium sulphate (MgSo 4.7H 2O), calcium chloride (CaCl2.2H 2O) and di-hydrogen potassium phosphate (KH2PO4) wer e 0.02%, 0.04% and 0.4% respectively.