SCREENING OF ANTIBACTERIAL ACTIVITY OF HYDROALCOHOLIC EXTRACT OF CYNODON DACTYLON

Abstract

Cynodon dactylon is a well-known plant used as a folk remedy in treatment of many symptoms and diseases like cramps, measles, tumors, wounds and warts. In this study the hydroalcoholic extract of Cynodon dactylon was investigated for their antibacterial activity against two gram positive bacteria (Staphylococcus aureus, Staphylococcus albus) and two gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) using agarwell diffusion method (zone of inhibition) and micro-dilution method (minimum inhibitory concentration). Ciprofloxacin was taken as positive control. Hydroalcoholic extract of Cynodon dactylon was taken as test sample and hydroalcoholic solvent was taken as negative control in a ratio of 60:40 of water and ethanol. From the results of zone of inhibition, it was concluded that hydroalcoholic extract of Cynodon dactylon possess an effective antibacterial activity while from results of minimum inhibitory concentration, it was revealed that all tested bacterial strains were sensitive towards Cynodon dactylon extract.