Cellular Distribution of Bioaccumulated Toxic Heavy Metals in Aspergillus Niger and Rhizopus Arrhizus

Abstract

A cadmium tolerant strain of Aspergillus niger and a lead tolerant strain of Rhizopus arrhizus were studied for distribution of accumulated toxic heavy metals in the mycelia in comparison to wild type strains. Accumulated cadmium in the cell wall fract ions of cadmium tolerant Aspergillus niger was more than the wild type strain. Cytosolic fraction conta ined the next highest load of metal. Similar was th e observation with lead tolerant Rhizopus arrhizus during bioaccumulation of lead. The results indica ted contribution of the surface property of the fungus in metal bioaccumulation. Involvement of the cellul ar metabolism during metal bioaccumulation and distribution in the sub-cellular compartments was substantiated by the use of some metabolic inhibito rs during growth in presence of metals. It was also observed that about 65% of the cell wall bound cadmium in Aspergillus niger and about 79% of the cell wall bound lead in Rhizopus arrhizus were recovered by Ethylene diamine tetra acetic aci d (EDTA) treatment, which indicated that the accumulated met als generally reside in the outer surface of the fu ngi, which could be recovered easily for possible commercial applications.