Rhamnogalacturonase B from Aspergillus aculeatus Is a Rhamnogalacturonan [alpha]-L-Rhamnopyranosyl-(1->4)-[alpha]-D-Galactopyranosyluronide Lyase

Abstract

The recently described rhamnogalacturonase B, which is able to degrade ramified hairy regions of pectin, was found to be a rhamnogalacturonan alpha-L-rhamnopyranosyl-(1-->4)-alpha-D-galactopyranosyluronide lyase. The cleavage site and mechanism differ from that of the previously described rhamnogalacturonase A, which is a hydrolase and can now be termed rhamnogalacturonan alpha-D-galactopyranosyluronide-(1-->2)-alpha-L-rhamnopyranosyl hydrolase.