Concepedia

Development of a Two-Step Multiplex PCR Assay for Typing of Capsular Polysaccharide Synthesis Gene Clusters of Streptococcus suis

Masatoshi Okura, Claude Lachance, Makoto Osaki, Tsutomu Sekizaki, Fumito Maruyama, Takashi Nozawa, Ichirô Nakagawa, Shigeyuki Hamada, Céline Rossignol, Marcelo Gottschalk,

Journal of Clinical Microbiology · 2014 · 141 citations · 20 references

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Concepts

Abstract

We developed a practical and easy two-step multiplex PCR assay to aid in serotyping of Streptococcus suis. The assay accurately typed almost all of the serotype reference strains and field isolates of various serotypes and also identified the genotypes of capsular polysaccharide synthesis gene clusters of some serologically nontypeable strains.

References

20

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Biochemical analysis, cpn60 and 16S rDNA sequence data indicate that Streptococcus suis serotypes 32 and 34, isolated from pigs, are Streptococcus orisratti

Janet E. Hill, Marcelo Gottschalk, Roland Brousseau et al. · Veterinary Microbiology · 2005 · 247 citations

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Description of 14 new capsular types of Streptococcus suis

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Some modifications in the procedure of direct sequencing of PCR amplified 16S rDNA

M. Dorsch, Erko Stackebrandt · Journal of Microbiological Methods · 1992 · 195 citations

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A polymerase chain reaction (PCR) assay specific for<i>Streptococcus suis</i>based on the gene encoding the glutamate dehydrogenase

Ogi Okwumabua, Michael B. O’Connor, Eileen Shull · FEMS Microbiology Letters · 2003 · 190 citations

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