In vitro combinatorial mutagenesis of the 65th and 222nd positions of the green fluorescent protein ofAequarea victoria

Functional antibody production using cell-free translation: Effects of protein disulfide isomerase and chaperones

Lyubov A. Ryabova, Dominique Desplancq, Alexander S. Spirin et al. · Nature Biotechnology · 1997 · 190 citations

Life Sciences, Immunology, Molecular Biology +16

Modifying the chain-length selectivity of the lipase from Burkholderia cepacia KWI-56 through in vitro combinatorial mutagenesis in the substrate-binding site

Junhao Yang, Yuichi Koga, Hideo Nakano et al. · Protein Engineering Design and Selection · 2002 · 85 citations · Full text

Life Sciences, Biochemistry, Microbiology +13

<i>In vitro</i> scanning saturation mutagenesis of an antibody binding pocket

Elizabeth A. Burks, Gang Chen, George Georgiou et al. · Proceedings of the National Academy of Sciences · 1997 · 75 citations · Full text

Pharmacy, Biotechnology, Molecular Biology +13

In vitro method for the generation of protein libraries using PCR amplification of a single DNA molecule and coupled transcription/translation

Shoji Ohuchi · Nucleic Acids Research · 1998 · 71 citations · Full text

Life Sciences, Biotechnology, Molecular Biology +17

Expression of Fab fragment of catalytic antibody 6D9 in an <i>Escherichia coli</i> in vitro coupled transcription/translation system

Xiuping Jiang, Yuji Ookubo, Ikuo Fujii et al. · FEBS Letters · 2002 · 63 citations · Full text

Life Sciences, Biochemistry, Molecular Biology +17