Covalent Fluorescence Labeling of His‐Tagged Proteins on the Surface of Living Cells

Abstract

Labeling His-tagged proteins on living cells. NTA-based covalent labeling reagents for His-tagged proteins were designed by combining the oligo-His directing effect of Ni-NTA with the irreversible reaction of a photocrosslinker. Using these new probes, a simple five-minute protocol for labeling His-tagged proteins on living cells was derived. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2268/2008/z800089_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.