Engineering a 2D Protein–DNA Crystal

Abstract

Weaving with DNA: A DNA-binding protein was used to control the structure of a self-assembled 2D crystal. In the absence of protein, four oligonucleotides hybridize to form a Kagome lattice of interwoven double helices with p3 symmetry (see image). Addition of protein RuvA during assembly changes the symmetry and connectivity to give a DNA–protein crystal with an approximately square unit cell. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2005/z463027_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.