Long‐Range Correlations between Aliphatic ¹³C Nuclei in Protein MAS NMR Spectroscopy

Abstract

Highly efficient polarization transfer can be achieved in the magic-angle spinning NMR analysis of proteins by the combination of 13C labeling at alternating positions and band-selective radio-frequency-driven recoupling (BASE RFDR), a pulse scheme aimed at exploiting the bandwidth selectivity and favorable effects of weak 13C radio-frequency irradiation to reintroduce the homonuclear dipolar interactions between distant nuclei.