Publication | Closed Access
AN ASSAY METHOD FOR HUMAN CHORIONIC GONADOTROPIN
49
Citations
0
References
1941
Year
FertilityReproductive HealthGynecologyFemale Reproductive FunctionImmature MouseReproductive BiologyEmbryologyReproductive EndocrinologyReproductive PhysiologyFemale InfertilityAssay MethodToxicologyReproductive MedicinePublic HealthInfertilitySatisfactory AssayEndocrinologyPharmacologyHuman ReproductionUterine ReceptivityPhysiologyMedicineEndocrine ResearchReproductive Hormone
SINCE the discovery of chorionic gonadotropic substance, a great variety of units and assay methods have been used by different workers for quantitative deter minations. The existence of so many methods is evidence in itself that none has been particularly satisfactory. The present work, previously published in abstract (I), was undertaken with the aim of devising an assay method which would have quantitative accuracy based upon objective data, and yet would eliminate the need for microscopic sections, i.e., a method which would be accurate and rapidly carried out, and simple enough to be used freely without prohibitive numbers of animals and excessive technical work. The increase in uterine size and fluid content as a secondary effect in immature rats and mice treated with gonadotropic substance has been observed by many workers (2–13). Levin and Tyndale (14), have used the uterine weight response in the immature mouse as a satisfactory assay for the gonadotropic substance from castrate urine, and Heller.