Publication | Closed Access
Analysis of Spontaneous Deletions and Gene Amplification in the lac Region of Escherichia coli
25
Citations
0
References
1983
Year
Gene AmplificationGeneticsBacteriologyBacteriophageMolecular BiologyEscherichia ColiGenomicsBacterial PathogensMicrobial EvolutionPhylogenetic AnalysisLaci GeneEvolutionary MicrobiologyLac RegionMolecular MicrobiologyNatural SciencesMicrobiologyMedicineGene Deletion DataSpontaneous RearrangementsMicrobial Genetics
Spontaneous rearrangements, such as large deletions and duplications, have important implications for the structure of the genome. It is therefore of great interest to analyze these events at the molecular level. We have previously described the distribution of spontaneous mutations in the lacI gene of Escherichia coli (Farabaugh et al. 1978). Approximately 15% of the observed I− mutations resulted from deletions. We analyzed 12 deletions at the DNA sequence level (Fig. 1) and found that 7 of these occurred at short repeats of 5 or 8 bp. All of the deletions examined arose in a recA+ background and ranged in length from 9 bp to 123 bp. Subsequently, deletions between short repeated sequences have been demonstrated in bacteriophage T7 (Studier et al. 1979), bacteriophage T4 (Pribnow et al. 1981), and E. coli (Brake et al. 1978; Fedoroff 1979; Ghosal and Saedler 1979; Ross et al. 1979; Post et al. 1980;...