A method for the control of the dissolved oxygen tension in microbial cultures

Abstract

Abstract A method for the control of dissolved oxygen tension in growing microbial cultures is described. The apparatus consists of a motor‐driven air sparge pipe which may be lowered or raised to give a variable point of entry of the air stream into the culture liquid and hence a variable gas dispersion and gas–liquid contact time. Control of the sparge pipe position is by means of a feedback control loop consisting of a dissolved oxygen probe, an on/off controller, and a reversing electric motor which drives the sparge pipe. The difficulty presented by the relatively slow response of the oxygen probe has been overcome by incorporating an adjustable rate of control action.