Objective

To replicate and extend recent findings in a Turkish population of associations between chronic rhinosinusitis (CRS) with nasal polyposis and single-nucleotide polymorphisms (SNPs) in theIL1A(rs17561 and Ser114Ala),IL1B(rs16944), andTNF(rs361525 and rs1800629) genes.

Design

In a case-control replication study, DNA samples were obtained from 206 patients with severe CRS (cases) and from 196 postal code–matched controls. ForIL1AandTNF, the 3 reported SNPs were complemented with tagging SNPs using an International HapMap genotyping data set to ensure complete genetic coverage. ForIL1B, only the single reported SNP was assessed. A total of 24 SNPs (7 inIL1A, 1 inIL1B, and 16 inTNF) were individually genotyped. The PLINK software package was used to perform genetic association tests.

Setting

Academic research.

Patients

Canadian population of individuals with severe CRS.

Main Outcome Measures

Allelic differences between cases and controls.

Results

Significant allelic differences between cases and controls were obtained forIL1Ars17561 (odds ratio [OR], 1.48;P = .02). The following 3 additional SNPs in this gene were associated with CRS: rs2856838 (OR, 0.63;P = .003), rs2048874 (OR, 0.57;P = .01), and rs1800587 (OR, 1.49;P = .02). These 3 SNPs remained significant after correction for multiple testing. No association was found withIL1BorTNF.

Conclusions

We replicated the previously reported association between theIL1Apolymorphism and severe CRS and identified 3 potential new associations in the same gene. This further supports the potential contribution ofIL1Ato the development of CRS. We were unable to replicate previous reports of associations withIL1BorTNF.

	Year	Citations

Abstract

References